Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (Canis lupus)

The discovery of novel probiotic bacteria from free-ranging animals for the treatment inflammatory bowel disease in domestic pets is a unique approach. chloroform extraction gastrointestinal (GI) tract material was used to inactivate vegetative cells and select spore-forming bacteria. A bacterium identified as Paenibacillus sp. strain via small ribosomal RNA (16S) gene sequencing isolated GI gray wolf (Canis lupus). typed Gram-variable, both catalase/oxidase-positive positive starch hydrolysis lipase assays. inhibited growth Staphylococcus aureus, Escherichia coli Micrococcus luteus. draft whole genome sequence (WGS) assembly 7,034,206 bp length, encoding 6543 genes, similar size coding capacity other closely related spp. isolate’s encodes several germination sporulation products along with antimicrobials such bacteriocin system chitinase. Enzyme genes alpha amylase, cellulase, lipases pectin lyase are also present genome. An incomplete lysogenic bacteriophage Phenotypic characteristics combined WGS genotype analysis indicate that this bacterium, designated ClWae2A, could be potential candidate dogs.